SEMKUR - Imaging
NIH/3T3 cells are plated with SEMKUR-Imaging at a 20 micromolar concentration and incubated with CO2 for 24 hours in complete media on a MatTek Dish. The media was then washed off and replaced with Hoechst, blue nuclear stain at a concentration of 1 microgram/ml. TMRM mitochondrial red stain was introduced at a concentration of 200nm while SEMKUR-Imaging green stain was used at a concentration of 20 micromolar. These stains were combined to show colocalization of the SEMKUR stain in the mitochondria.